Loop mediated isothermal amplification (LAMP): a new generation of innovative gene amplification technique; perspectives in clinical diagnosis of infectious diseases
Identifieur interne : 003333 ( Main/Exploration ); précédent : 003332; suivant : 003334Loop mediated isothermal amplification (LAMP): a new generation of innovative gene amplification technique; perspectives in clinical diagnosis of infectious diseases
Auteurs : Manmohan Parida [Inde] ; Santhosh Sannarangaiah [Inde] ; Paban Kumar Dash [Inde] ; P. V. L. Rao [Inde] ; Kouichi Morita [Japon]Source :
- Reviews in Medical Virology [ 1052-9276 ] ; 2008-11.
English descriptors
- Teeft :
- Amplif, Amplification, Assay, Clin, Clin microbiol, Clinical diagnosis, Colour, Colour change, Complementary strand, Constant temperature, Copyright, Cycling, Distinct regions, Eiken chemical, Gene, Gene copy number, Gene method, Herpesvirus, Human herpesvirus, Ication, Ihira, Internal primer, Internal primers, Isothermal, Isothermal amplification amplif ication, Isothermal assay, Isothermal method, John wiley sons, Lamp, Lamp amplification, Lamp assay, Lamp cycling, Lamp method, Lamp product, Lamp reaction, Loop primer, Loop primers, Loopmediated, Magnesium pyrophosphate, Microbiol, Nucleic, Nucleotide, Outer primer, Parida, Polymerase, Positivity, Primer, Primer design, Pyrophosphate, Rapid detection, Rapid diagnosis, Reaction mixture, Same strand, Single nucleotide difference, Single step, Single strand, Single tube, Standard curve, Strand, Strand displacement, Strand displacement activity, Target gene, Target sequence, Thermal cycler, Transcription, Transcription loop, Turbidity, Uorescent, Virol, Virol methods, Virus, Visual turbidity, Yoshikawa.
Abstract
Loop mediated isothermal amplification (LAMP) is a powerful innovative gene amplification technique emerging as a simple rapid diagnostic tool for early detection and identification of microbial diseases. The whole procedure is very simple and rapid wherein the amplification can be completed in less than 1 h under isothermal conditions employing a set of six specially designed primers spanning eight distinct sequences of a target gene, by incubating all the reagents in a single tube. Gene amplification products can be detected by agarose gel electrophoresis as well as by real‐time monitoring in an inexpensive turbidimeter. Gene copy number can also be quantified with the help of a standard curve generated from different concentrations of gene copy number plotted against time of positivity with the help of a real‐time turbidimeter. Alternatively, gene amplification can be visualised by the naked eye either as turbidity or in the form of a colour change when SYBR Green I, a fluorescent dsDNA intercalating dye, is employed. LAMP does not require a thermal cycler and can be performed simply with a heating block and/or water bath. Considering the advantages of rapid amplification, simple operation and easy detection, LAMP has potential applications for clinical diagnosis as well as surveillance of infectious diseases in developing countries without requiring sophisticated equipment or skilled personnel. Copyright © 2008 John Wiley & Sons, Ltd.
Url:
DOI: 10.1002/rmv.593
Affiliations:
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The whole procedure is very simple and rapid wherein the amplification can be completed in less than 1 h under isothermal conditions employing a set of six specially designed primers spanning eight distinct sequences of a target gene, by incubating all the reagents in a single tube. Gene amplification products can be detected by agarose gel electrophoresis as well as by real‐time monitoring in an inexpensive turbidimeter. Gene copy number can also be quantified with the help of a standard curve generated from different concentrations of gene copy number plotted against time of positivity with the help of a real‐time turbidimeter. Alternatively, gene amplification can be visualised by the naked eye either as turbidity or in the form of a colour change when SYBR Green I, a fluorescent dsDNA intercalating dye, is employed. LAMP does not require a thermal cycler and can be performed simply with a heating block and/or water bath. Considering the advantages of rapid amplification, simple operation and easy detection, LAMP has potential applications for clinical diagnosis as well as surveillance of infectious diseases in developing countries without requiring sophisticated equipment or skilled personnel. Copyright © 2008 John Wiley & Sons, Ltd.</div></front></TEI><affiliations><list><country><li>Inde</li><li>Japon</li></country></list><tree><country name="Inde"><noRegion><name sortKey="Parida, Manmohan" sort="Parida, Manmohan" uniqKey="Parida M" first="Manmohan" last="Parida">Manmohan Parida</name></noRegion><name sortKey="Dash, Paban Kumar" sort="Dash, Paban Kumar" uniqKey="Dash P" first="Paban Kumar" last="Dash">Paban Kumar Dash</name><name sortKey="Parida, Manmohan" sort="Parida, Manmohan" uniqKey="Parida M" first="Manmohan" last="Parida">Manmohan Parida</name><name sortKey="Rao, P V L" sort="Rao, P V L" uniqKey="Rao P" first="P. V. L." last="Rao">P. V. L. Rao</name><name sortKey="Sannarangaiah, Santhosh" sort="Sannarangaiah, Santhosh" uniqKey="Sannarangaiah S" first="Santhosh" last="Sannarangaiah">Santhosh Sannarangaiah</name></country><country name="Japon"><noRegion><name sortKey="Morita, Kouichi" sort="Morita, Kouichi" uniqKey="Morita K" first="Kouichi" last="Morita">Kouichi Morita</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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